A novel protocol of whole mount electro-immunofluorescence staining

PURPOSE: To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. METHODS: Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde and 0.2% g...

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Detalles Bibliográficos
Autores principales: Liu, Hongshan, Kao, Winston W.Y.
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650717/
https://www.ncbi.nlm.nih.gov/pubmed/19262742

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650717/
https://www.ncbi.nlm.nih.gov/pubmed/19262742

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