Real-time single-molecule observation of rolling-circle DNA replication

We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities...

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Detalles Bibliográficos
Autores principales: Tanner, Nathan A., Loparo, Joseph J., Hamdan, Samir M., Jergic, Slobodan, Dixon, Nicholas E., van Oijen, Antoine M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651787/
https://www.ncbi.nlm.nih.gov/pubmed/19155275
http://dx.doi.org/10.1093/nar/gkp006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651787/
https://www.ncbi.nlm.nih.gov/pubmed/19155275
http://dx.doi.org/10.1093/nar/gkp006

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