Using multiplexed regulation of luciferase activity and GFP translocation to screen for FOXO modulators

BACKGROUND: Independent luciferase reporter assays and fluorescent translocation assays have been successfully used in drug discovery for several molecular targets. We developed U2transLUC, an assay system in which luciferase and fluorescent read-outs can be multiplexed to provide a powerful cell-ba...

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Detalles Bibliográficos
Autores principales: Zanella, Fabian, Rosado, Aranzazú, Garcia, Beatriz, Carnero, Amancio, Link, Wolfgang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651847/
https://www.ncbi.nlm.nih.gov/pubmed/19243599
http://dx.doi.org/10.1186/1471-2121-10-14

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651847/
https://www.ncbi.nlm.nih.gov/pubmed/19243599
http://dx.doi.org/10.1186/1471-2121-10-14

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