DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes

We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repress...

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Detalles Bibliográficos
Autores principales: Butala, Matej, Busby, Stephen J. W., Lee, David J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655658/
https://www.ncbi.nlm.nih.gov/pubmed/19181705
http://dx.doi.org/10.1093/nar/gkp043

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655658/
https://www.ncbi.nlm.nih.gov/pubmed/19181705
http://dx.doi.org/10.1093/nar/gkp043

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