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Diene-modified nucleotides for the Diels–Alder-mediated functional tagging of DNA

We explore the potential of the Diels–Alder cycloaddition for the functional tagging of DNA strands. A deoxyuridine triphosphate derivative carrying a diene at position 5 of the pyrimidine base was synthesized using a two-step procedure. The derivative was efficiently accepted as substrate in enzyma...

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Detalles Bibliográficos
Autores principales: Borsenberger, Vinciane, Howorka, Stefan
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655660/
https://www.ncbi.nlm.nih.gov/pubmed/19139071
http://dx.doi.org/10.1093/nar/gkn1066