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Solution Hybrid Selection with Ultra-long Oligonucleotides for Massively Parallel Targeted Sequencing
Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA “baits” to “fish” targets out of a “pond” of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides origi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663421/ https://www.ncbi.nlm.nih.gov/pubmed/19182786 http://dx.doi.org/10.1038/nbt.1523 |