Solution Hybrid Selection with Ultra-long Oligonucleotides for Massively Parallel Targeted Sequencing

Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA “baits” to “fish” targets out of a “pond” of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides origi...

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Detalles Bibliográficos
Autores principales: Gnirke, Andreas, Melnikov, Alexandre, Maguire, Jared, Rogov, Peter, LeProust, Emily M., Brockman, William, Fennell, Timothy, Giannoukos, Georgia, Fisher, Sheila, Russ, Carsten, Gabriel, Stacey, Jaffe, David B., Lander, Eric S., Nusbaum, Chad
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663421/
https://www.ncbi.nlm.nih.gov/pubmed/19182786
http://dx.doi.org/10.1038/nbt.1523

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