Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes

Yersinia pestis proteins were sequentially extracted from crude membranes with a high salt buffer (2.5 M NaBr), an alkaline solution (180 mM Na(2)CO(3), pH 11.3) and membrane denaturants (8 M urea, 2 M thiourea and 1% amidosulfobetaine-14). Separation of proteins by 2D gel electrophoresis was follow...

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Detalles Bibliográficos
Autores principales: Pieper, Rembert, Huang, Shih-Ting, Clark, David J, Robinson, Jeffrey M, Alami, Hamid, Parmar, Prashanth P, Suh, Moo-Jin, Kuntumalla, Srilatha, Bunai, Christine L, Perry, Robert D, Fleischmann, Robert D, Peterson, Scott N
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663777/
https://www.ncbi.nlm.nih.gov/pubmed/19228400
http://dx.doi.org/10.1186/1477-5956-7-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663777/
https://www.ncbi.nlm.nih.gov/pubmed/19228400
http://dx.doi.org/10.1186/1477-5956-7-5

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