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Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes
Yersinia pestis proteins were sequentially extracted from crude membranes with a high salt buffer (2.5 M NaBr), an alkaline solution (180 mM Na(2)CO(3), pH 11.3) and membrane denaturants (8 M urea, 2 M thiourea and 1% amidosulfobetaine-14). Separation of proteins by 2D gel electrophoresis was follow...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663777/ https://www.ncbi.nlm.nih.gov/pubmed/19228400 http://dx.doi.org/10.1186/1477-5956-7-5 |