Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of GC-biased genomes

Amplification artifacts introduced during library preparation for the Illumina Genome Analyzer increase the likelihood that an appreciable proportion of these sequences will be duplicates, and cause an uneven distribution of read coverage across the targeted sequencing regions. As a consequence, the...

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Detalles Bibliográficos
Autores principales: Kozarewa, Iwanka, Ning, Zemin, Quail, Michael A., Sanders, Mandy J., Berriman, Matthew, Turner, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664327/
https://www.ncbi.nlm.nih.gov/pubmed/19287394
http://dx.doi.org/10.1038/nmeth.1311

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664327/
https://www.ncbi.nlm.nih.gov/pubmed/19287394
http://dx.doi.org/10.1038/nmeth.1311

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