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Copper(II) Binding to α-Synuclein, the Parkinson’s Protein
[Image: see text] Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with α-synuclein, a protein implicated in Parkinson’s disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (K(d) ∼ 100 nM) near the N-terminus at pH...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664836/ https://www.ncbi.nlm.nih.gov/pubmed/18465859 http://dx.doi.org/10.1021/ja711415b |
| _version_ | 1782165998760099840 |
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| author | Lee, Jennifer C. Gray, Harry B. Winkler, Jay R. |
| author_facet | Lee, Jennifer C. Gray, Harry B. Winkler, Jay R. |
| author_sort | Lee, Jennifer C. |
| collection | PubMed |
| description | [Image: see text] Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with α-synuclein, a protein implicated in Parkinson’s disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (K(d) ∼ 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site. |
| format | Text |
| id | pubmed-2664836 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2008 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-26648362009-04-06 Copper(II) Binding to α-Synuclein, the Parkinson’s Protein Lee, Jennifer C. Gray, Harry B. Winkler, Jay R. J Am Chem Soc [Image: see text] Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with α-synuclein, a protein implicated in Parkinson’s disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (K(d) ∼ 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site. American Chemical Society 2008-05-09 2008-06-04 /pmc/articles/PMC2664836/ /pubmed/18465859 http://dx.doi.org/10.1021/ja711415b Text en Copyright © 2008 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org. 40.75 |
| spellingShingle | Lee, Jennifer C. Gray, Harry B. Winkler, Jay R. Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title | Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title_full | Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title_fullStr | Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title_full_unstemmed | Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title_short | Copper(II) Binding to α-Synuclein, the Parkinson’s Protein |
| title_sort | copper(ii) binding to α-synuclein, the parkinson’s protein |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664836/ https://www.ncbi.nlm.nih.gov/pubmed/18465859 http://dx.doi.org/10.1021/ja711415b |
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