Comparison of non-viral methods to genetically modify and enrich populations of primary human corneal endothelial cells

PURPOSE: To compare different techniques of transfection of primary human corneal endothelial cells (HCECs) by non-viral methods and to enrich genetically modified cells to a highly pure population. METHODS: HCECs were cultured following previously published methods. Dissection of the Descemet membr...

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Detalles Bibliográficos
Autores principales: Engler, Christoph, Kelliher, Clare, Wahlin, Karl J., Speck, Caroline L., Jun, Albert S.
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664841/
https://www.ncbi.nlm.nih.gov/pubmed/19347048

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664841/
https://www.ncbi.nlm.nih.gov/pubmed/19347048

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