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In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins
BACKGROUND: Protein trans-splicing by naturally occurring split DnaE inteins is used for protein ligation of foreign peptide fragments. In order to widen biotechnological applications of protein trans-splicing, it is highly desirable to have split inteins with shorter C-terminal fragments, which can...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664965/ https://www.ncbi.nlm.nih.gov/pubmed/19365564 http://dx.doi.org/10.1371/journal.pone.0005185 |