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In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

BACKGROUND: Protein trans-splicing by naturally occurring split DnaE inteins is used for protein ligation of foreign peptide fragments. In order to widen biotechnological applications of protein trans-splicing, it is highly desirable to have split inteins with shorter C-terminal fragments, which can...

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Detalles Bibliográficos
Autores principales: Aranko, A. Sesilja, Züger, Sara, Buchinger, Edith, Iwaï, Hideo
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664965/
https://www.ncbi.nlm.nih.gov/pubmed/19365564
http://dx.doi.org/10.1371/journal.pone.0005185