Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence baseline. This article shows that baseline es...

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Detalles Bibliográficos
Autores principales: Ruijter, J. M., Ramakers, C., Hoogaars, W. M. H., Karlen, Y., Bakker, O., van den Hoff, M. J. B., Moorman, A. F. M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665230/
https://www.ncbi.nlm.nih.gov/pubmed/19237396
http://dx.doi.org/10.1093/nar/gkp045

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665230/
https://www.ncbi.nlm.nih.gov/pubmed/19237396
http://dx.doi.org/10.1093/nar/gkp045

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