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Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence baseline. This article shows that baseline es...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665230/ https://www.ncbi.nlm.nih.gov/pubmed/19237396 http://dx.doi.org/10.1093/nar/gkp045 |