U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro....

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Autores principales: Fukumura, Kazuhiro, Taniguchi, Ichiro, Sakamoto, Hiroshi, Ohno, Mutsuhito, Inoue, Kunio
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665233/
https://www.ncbi.nlm.nih.gov/pubmed/19190090
http://dx.doi.org/10.1093/nar/gkp050
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author Fukumura, Kazuhiro
Taniguchi, Ichiro
Sakamoto, Hiroshi
Ohno, Mutsuhito
Inoue, Kunio
author_facet Fukumura, Kazuhiro
Taniguchi, Ichiro
Sakamoto, Hiroshi
Ohno, Mutsuhito
Inoue, Kunio
author_sort Fukumura, Kazuhiro
collection PubMed
description U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
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spelling pubmed-26652332009-04-06 U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing Fukumura, Kazuhiro Taniguchi, Ichiro Sakamoto, Hiroshi Ohno, Mutsuhito Inoue, Kunio Nucleic Acids Res RNA U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs. Oxford University Press 2009-04 2009-02-03 /pmc/articles/PMC2665233/ /pubmed/19190090 http://dx.doi.org/10.1093/nar/gkp050 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Fukumura, Kazuhiro
Taniguchi, Ichiro
Sakamoto, Hiroshi
Ohno, Mutsuhito
Inoue, Kunio
U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title_full U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title_fullStr U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title_full_unstemmed U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title_short U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
title_sort u1-independent pre-mrna splicing contributes to the regulation of alternative splicing
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665233/
https://www.ncbi.nlm.nih.gov/pubmed/19190090
http://dx.doi.org/10.1093/nar/gkp050
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