Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing

Direct cloning and parallel sequencing, an extremely powerful method for microRNA (miRNA) discovery, has not yet been applied to bacterial transcriptomes. Here we present sRNA-Seq, an unbiased method that allows for interrogation of the entire small, non-coding RNA (sRNA) repertoire in any prokaryot...

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Detalles Bibliográficos
Autores principales: Liu, Jane M., Livny, Jonathan, Lawrence, Michael S., Kimball, Marc D., Waldor, Matthew K., Camilli, Andrew
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665243/
https://www.ncbi.nlm.nih.gov/pubmed/19223322
http://dx.doi.org/10.1093/nar/gkp080

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665243/
https://www.ncbi.nlm.nih.gov/pubmed/19223322
http://dx.doi.org/10.1093/nar/gkp080

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