Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis

Herein we present a simple, cost-effective TopDown (TD) gene synthesis method that eliminates the interference between the polymerase chain reactions (PCR) assembly and amplification in one-step gene synthesis. The method involves two key steps: (i) design of outer primers and assembly oligonucleoti...

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Detalles Bibliográficos
Autores principales: Ye, Hongye, Huang, Mo Chao, Li, Mo-Huang, Ying, Jackie Y.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673447/
https://www.ncbi.nlm.nih.gov/pubmed/19264797
http://dx.doi.org/10.1093/nar/gkp118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673447/
https://www.ncbi.nlm.nih.gov/pubmed/19264797
http://dx.doi.org/10.1093/nar/gkp118

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