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Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis

Herein we present a simple, cost-effective TopDown (TD) gene synthesis method that eliminates the interference between the polymerase chain reactions (PCR) assembly and amplification in one-step gene synthesis. The method involves two key steps: (i) design of outer primers and assembly oligonucleoti...

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Detalles Bibliográficos
Autores principales:	Ye, Hongye, Huang, Mo Chao, Li, Mo-Huang, Ying, Jackie Y.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673447/ https://www.ncbi.nlm.nih.gov/pubmed/19264797 http://dx.doi.org/10.1093/nar/gkp118

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