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Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673709/ https://www.ncbi.nlm.nih.gov/pubmed/17486136 http://dx.doi.org/10.1038/msb4100150 |
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author | Oron, Efrat Tuller, Tamir Li, Ling Rozovsky, Nina Yekutieli, Daniel Rencus-Lazar, Sigal Segal, Daniel Chor, Benny Edgar, Bruce A Chamovitz, Daniel A |
author_facet | Oron, Efrat Tuller, Tamir Li, Ling Rozovsky, Nina Yekutieli, Daniel Rencus-Lazar, Sigal Segal, Daniel Chor, Benny Edgar, Bruce A Chamovitz, Daniel A |
author_sort | Oron, Efrat |
collection | PubMed |
description | The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this important complex. Transcriptome data were generated for four csn mutants, sampled at three developmental time points. Our results are highly reproducible, being confirmed using two different experimental setups that entail different microarrays and different controls. Our results indicate that the CSN acts as a transcriptional repressor during development of Drosophila, resulting in achronic gene expression in the csn mutants. ‘Time shift' analysis with the publicly available Drosophila transcriptome data indicates that genes repressed by the CSN are normally induced primarily during late embryogenesis or during metamorphosis. These temporal shifts are likely due to the roles of the CSN in regulating transcription factors. A null mutation in CSN subunit 4 and hypomorphic mutations in csn5 lead to more severe defects than seen in the csn5-null mutants strain, suggesting that CSN5 carries only some of the CSN function. |
format | Text |
id | pubmed-2673709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-26737092009-04-28 Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression Oron, Efrat Tuller, Tamir Li, Ling Rozovsky, Nina Yekutieli, Daniel Rencus-Lazar, Sigal Segal, Daniel Chor, Benny Edgar, Bruce A Chamovitz, Daniel A Mol Syst Biol Article The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this important complex. Transcriptome data were generated for four csn mutants, sampled at three developmental time points. Our results are highly reproducible, being confirmed using two different experimental setups that entail different microarrays and different controls. Our results indicate that the CSN acts as a transcriptional repressor during development of Drosophila, resulting in achronic gene expression in the csn mutants. ‘Time shift' analysis with the publicly available Drosophila transcriptome data indicates that genes repressed by the CSN are normally induced primarily during late embryogenesis or during metamorphosis. These temporal shifts are likely due to the roles of the CSN in regulating transcription factors. A null mutation in CSN subunit 4 and hypomorphic mutations in csn5 lead to more severe defects than seen in the csn5-null mutants strain, suggesting that CSN5 carries only some of the CSN function. Nature Publishing Group 2007-05-08 /pmc/articles/PMC2673709/ /pubmed/17486136 http://dx.doi.org/10.1038/msb4100150 Text en Copyright © 2007, EMBO and Nature Publishing Group http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Article Oron, Efrat Tuller, Tamir Li, Ling Rozovsky, Nina Yekutieli, Daniel Rencus-Lazar, Sigal Segal, Daniel Chor, Benny Edgar, Bruce A Chamovitz, Daniel A Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title | Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title_full | Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title_fullStr | Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title_full_unstemmed | Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title_short | Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression |
title_sort | genomic analysis of cop9 signalosome function in drosophila melanogaster reveals a role in temporal regulation of gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673709/ https://www.ncbi.nlm.nih.gov/pubmed/17486136 http://dx.doi.org/10.1038/msb4100150 |
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