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Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression

The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this...

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Autores principales: Oron, Efrat, Tuller, Tamir, Li, Ling, Rozovsky, Nina, Yekutieli, Daniel, Rencus-Lazar, Sigal, Segal, Daniel, Chor, Benny, Edgar, Bruce A, Chamovitz, Daniel A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673709/
https://www.ncbi.nlm.nih.gov/pubmed/17486136
http://dx.doi.org/10.1038/msb4100150
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author Oron, Efrat
Tuller, Tamir
Li, Ling
Rozovsky, Nina
Yekutieli, Daniel
Rencus-Lazar, Sigal
Segal, Daniel
Chor, Benny
Edgar, Bruce A
Chamovitz, Daniel A
author_facet Oron, Efrat
Tuller, Tamir
Li, Ling
Rozovsky, Nina
Yekutieli, Daniel
Rencus-Lazar, Sigal
Segal, Daniel
Chor, Benny
Edgar, Bruce A
Chamovitz, Daniel A
author_sort Oron, Efrat
collection PubMed
description The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this important complex. Transcriptome data were generated for four csn mutants, sampled at three developmental time points. Our results are highly reproducible, being confirmed using two different experimental setups that entail different microarrays and different controls. Our results indicate that the CSN acts as a transcriptional repressor during development of Drosophila, resulting in achronic gene expression in the csn mutants. ‘Time shift' analysis with the publicly available Drosophila transcriptome data indicates that genes repressed by the CSN are normally induced primarily during late embryogenesis or during metamorphosis. These temporal shifts are likely due to the roles of the CSN in regulating transcription factors. A null mutation in CSN subunit 4 and hypomorphic mutations in csn5 lead to more severe defects than seen in the csn5-null mutants strain, suggesting that CSN5 carries only some of the CSN function.
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spelling pubmed-26737092009-04-28 Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression Oron, Efrat Tuller, Tamir Li, Ling Rozovsky, Nina Yekutieli, Daniel Rencus-Lazar, Sigal Segal, Daniel Chor, Benny Edgar, Bruce A Chamovitz, Daniel A Mol Syst Biol Article The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin–proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this important complex. Transcriptome data were generated for four csn mutants, sampled at three developmental time points. Our results are highly reproducible, being confirmed using two different experimental setups that entail different microarrays and different controls. Our results indicate that the CSN acts as a transcriptional repressor during development of Drosophila, resulting in achronic gene expression in the csn mutants. ‘Time shift' analysis with the publicly available Drosophila transcriptome data indicates that genes repressed by the CSN are normally induced primarily during late embryogenesis or during metamorphosis. These temporal shifts are likely due to the roles of the CSN in regulating transcription factors. A null mutation in CSN subunit 4 and hypomorphic mutations in csn5 lead to more severe defects than seen in the csn5-null mutants strain, suggesting that CSN5 carries only some of the CSN function. Nature Publishing Group 2007-05-08 /pmc/articles/PMC2673709/ /pubmed/17486136 http://dx.doi.org/10.1038/msb4100150 Text en Copyright © 2007, EMBO and Nature Publishing Group http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Article
Oron, Efrat
Tuller, Tamir
Li, Ling
Rozovsky, Nina
Yekutieli, Daniel
Rencus-Lazar, Sigal
Segal, Daniel
Chor, Benny
Edgar, Bruce A
Chamovitz, Daniel A
Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title_full Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title_fullStr Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title_full_unstemmed Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title_short Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression
title_sort genomic analysis of cop9 signalosome function in drosophila melanogaster reveals a role in temporal regulation of gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673709/
https://www.ncbi.nlm.nih.gov/pubmed/17486136
http://dx.doi.org/10.1038/msb4100150
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