Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization

The effects of photocaged nucleosides on the DNA polymerization reaction was investigated, finding that most polymerases are unable to recognize and read through the presence of a single caging group on the DNA template. Based on this discovery, a new method of introducing mutations into plasmid DNA...

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Detalles Bibliográficos
Autores principales: Young, Douglas D., Lusic, Hrvoje, Lively, Mark O., Deiters, Alexander
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677887/
https://www.ncbi.nlm.nih.gov/pubmed/19293272
http://dx.doi.org/10.1093/nar/gkp150

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677887/
https://www.ncbi.nlm.nih.gov/pubmed/19293272
http://dx.doi.org/10.1093/nar/gkp150

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