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Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
The effects of photocaged nucleosides on the DNA polymerization reaction was investigated, finding that most polymerases are unable to recognize and read through the presence of a single caging group on the DNA template. Based on this discovery, a new method of introducing mutations into plasmid DNA...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677887/ https://www.ncbi.nlm.nih.gov/pubmed/19293272 http://dx.doi.org/10.1093/nar/gkp150 |