Novel method for high-throughput colony PCR screening in nanoliter-reactors

We introduce a technology for the rapid identification and sequencing of conserved DNA elements employing a novel suspension array based on nanoliter (nl)-reactors made from alginate. The reactors have a volume of 35 nl and serve as reaction compartments during monoseptic growth of microbial library...

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Detalles Bibliográficos
Autores principales: Walser, Marcel, Pellaux, Rene, Meyer, Andreas, Bechtold, Matthias, Vanderschuren, Herve, Reinhardt, Richard, Magyar, Joseph, Panke, Sven, Held, Martin
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677890/
https://www.ncbi.nlm.nih.gov/pubmed/19282448
http://dx.doi.org/10.1093/nar/gkp160

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677890/
https://www.ncbi.nlm.nih.gov/pubmed/19282448
http://dx.doi.org/10.1093/nar/gkp160

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