Cargando…
Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury
BACKGROUND: It is widely believed that atherosclerotic plaque rupture and subsequent thrombosis leads to acute coronary events and stroke. However, study of the mechanism and treatment of human plaque rupture is hampered by lack of a suitable animal model. Our aim was to develop a novel animal model...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2682487/ https://www.ncbi.nlm.nih.gov/pubmed/19344521 http://dx.doi.org/10.1186/1423-0127-16-39 |
_version_ | 1782167059709296640 |
---|---|
author | Fang, Shun-Miao Zhang, Qing-Hua Jiang, Zhi-Xin |
author_facet | Fang, Shun-Miao Zhang, Qing-Hua Jiang, Zhi-Xin |
author_sort | Fang, Shun-Miao |
collection | PubMed |
description | BACKGROUND: It is widely believed that atherosclerotic plaque rupture and subsequent thrombosis leads to acute coronary events and stroke. However, study of the mechanism and treatment of human plaque rupture is hampered by lack of a suitable animal model. Our aim was to develop a novel animal model of atherosclerotic plaque rupture to facilitate the study of human plaque disruption and thrombosis. METHODS: 28 healthy male New Zealand white rabbits were randomly divided into two groups: rabbits in group A (n = 12) were only fed a high-fat diet for eight weeks; rabbits in group B (n = 16) underwent cold-induced endothelial injury with liquid nitrogen, then were given a high-fat diet for eight weeks. After completion of the preparatory regimen, triggering of plaque rupture was attempted by local injection of liquid nitrogen in both groups. RESULTS: All rabbits in group B had disrupted plaques or rupture-driven occlusive thrombus formation, but none in group A showed any effects. More importantly, the cold-induced plaques in our model were reminiscent of human atherosclerotic plaques in terms of architecture, cellular composition, growth characteristics, and patterns of lipid accumulation. CONCLUSION: We successfully developed a novel rabbit model of atherosclerotic plaque rupture and thrombosis, which is simple, fast, inexpensive, and reproducible, and has a low mortality and a high yield of triggering. This model will allow us to better understand the mechanism of human plaque rupture and also to develop plaque-stabilizing therapies. |
format | Text |
id | pubmed-2682487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26824872009-05-15 Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury Fang, Shun-Miao Zhang, Qing-Hua Jiang, Zhi-Xin J Biomed Sci Research BACKGROUND: It is widely believed that atherosclerotic plaque rupture and subsequent thrombosis leads to acute coronary events and stroke. However, study of the mechanism and treatment of human plaque rupture is hampered by lack of a suitable animal model. Our aim was to develop a novel animal model of atherosclerotic plaque rupture to facilitate the study of human plaque disruption and thrombosis. METHODS: 28 healthy male New Zealand white rabbits were randomly divided into two groups: rabbits in group A (n = 12) were only fed a high-fat diet for eight weeks; rabbits in group B (n = 16) underwent cold-induced endothelial injury with liquid nitrogen, then were given a high-fat diet for eight weeks. After completion of the preparatory regimen, triggering of plaque rupture was attempted by local injection of liquid nitrogen in both groups. RESULTS: All rabbits in group B had disrupted plaques or rupture-driven occlusive thrombus formation, but none in group A showed any effects. More importantly, the cold-induced plaques in our model were reminiscent of human atherosclerotic plaques in terms of architecture, cellular composition, growth characteristics, and patterns of lipid accumulation. CONCLUSION: We successfully developed a novel rabbit model of atherosclerotic plaque rupture and thrombosis, which is simple, fast, inexpensive, and reproducible, and has a low mortality and a high yield of triggering. This model will allow us to better understand the mechanism of human plaque rupture and also to develop plaque-stabilizing therapies. BioMed Central 2009-04-04 /pmc/articles/PMC2682487/ /pubmed/19344521 http://dx.doi.org/10.1186/1423-0127-16-39 Text en Copyright © 2009 Fang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Fang, Shun-Miao Zhang, Qing-Hua Jiang, Zhi-Xin Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title | Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title_full | Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title_fullStr | Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title_full_unstemmed | Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title_short | Developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
title_sort | developing a novel rabbit model of atherosclerotic plaque rupture and thrombosis by cold-induced endothelial injury |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2682487/ https://www.ncbi.nlm.nih.gov/pubmed/19344521 http://dx.doi.org/10.1186/1423-0127-16-39 |
work_keys_str_mv | AT fangshunmiao developinganovelrabbitmodelofatheroscleroticplaqueruptureandthrombosisbycoldinducedendothelialinjury AT zhangqinghua developinganovelrabbitmodelofatheroscleroticplaqueruptureandthrombosisbycoldinducedendothelialinjury AT jiangzhixin developinganovelrabbitmodelofatheroscleroticplaqueruptureandthrombosisbycoldinducedendothelialinjury |