Accurate characterization of weak macromolecular interactions by titration of NMR residual dipolar couplings: application to the CD2AP SH3-C:ubiquitin complex

The description of the interactome represents one of key challenges remaining for structural biology. Physiologically important weak interactions, with dissociation constants above 100 μM, are remarkably common, but remain beyond the reach of most of structural biology. NMR spectroscopy, and in part...

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Detalles Bibliográficos
Autores principales: Ortega-Roldan, Jose Luis, Jensen, Malene Ringkjøbing, Brutscher, Bernhard, Azuaga, Ana I., Blackledge, Martin, van Nuland, Nico A. J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685109/
https://www.ncbi.nlm.nih.gov/pubmed/19359362
http://dx.doi.org/10.1093/nar/gkp211

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685109/
https://www.ncbi.nlm.nih.gov/pubmed/19359362
http://dx.doi.org/10.1093/nar/gkp211

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