Cargando…

The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release

The P2X(7) receptor (P2X(7)R) is uniquely associated with two distinct cellular responses: activation of a dye-permeable pathway allowing passage of molecules up to 900 Da and rapid release of the pro-inflammatory cytokine, interleukin-1β (IL-1β), from activated macrophage. How this dye uptake path...

Descripción completa

Detalles Bibliográficos
Autores principales: Pelegrin, Pablo, Surprenant, Annmarie
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686830/
https://www.ncbi.nlm.nih.gov/pubmed/19212823
http://dx.doi.org/10.1007/s11302-009-9141-7
_version_ 1782167477776547840
author Pelegrin, Pablo
Surprenant, Annmarie
author_facet Pelegrin, Pablo
Surprenant, Annmarie
author_sort Pelegrin, Pablo
collection PubMed
description The P2X(7) receptor (P2X(7)R) is uniquely associated with two distinct cellular responses: activation of a dye-permeable pathway allowing passage of molecules up to 900 Da and rapid release of the pro-inflammatory cytokine, interleukin-1β (IL-1β), from activated macrophage. How this dye uptake path forms and whether it is involved in IL-1β release has not been known. Pannexin-1 is a recently identified protein found to physically associate with the P2X(7)R. Inhibition of pannexin-1 does not alter P2X(7)R ion channel activation or associated calcium flux but blocks one component of P2X(7)R-induced dye uptake and unmasks a slower, previously undetected, dye uptake pathway. Inhibition of pannexin-1 blocks P2X(7)R-mediated IL-1β release from macrophage as well as release mediated by other stimuli which couple to activation of capase-1 and additionally inhibits the release of interleukin-1α, a member of the IL-1 family whose processing does not require caspase-1 activation. Thus, pannexin-1 is linked to both dye uptake and IL-1β release but via distinct mechanisms.
format Text
id pubmed-2686830
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-26868302009-06-08 The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release Pelegrin, Pablo Surprenant, Annmarie Purinergic Signal Review The P2X(7) receptor (P2X(7)R) is uniquely associated with two distinct cellular responses: activation of a dye-permeable pathway allowing passage of molecules up to 900 Da and rapid release of the pro-inflammatory cytokine, interleukin-1β (IL-1β), from activated macrophage. How this dye uptake path forms and whether it is involved in IL-1β release has not been known. Pannexin-1 is a recently identified protein found to physically associate with the P2X(7)R. Inhibition of pannexin-1 does not alter P2X(7)R ion channel activation or associated calcium flux but blocks one component of P2X(7)R-induced dye uptake and unmasks a slower, previously undetected, dye uptake pathway. Inhibition of pannexin-1 blocks P2X(7)R-mediated IL-1β release from macrophage as well as release mediated by other stimuli which couple to activation of capase-1 and additionally inhibits the release of interleukin-1α, a member of the IL-1 family whose processing does not require caspase-1 activation. Thus, pannexin-1 is linked to both dye uptake and IL-1β release but via distinct mechanisms. Springer Netherlands 2009-02-12 2009-06 /pmc/articles/PMC2686830/ /pubmed/19212823 http://dx.doi.org/10.1007/s11302-009-9141-7 Text en © The Author(s) 2009
spellingShingle Review
Pelegrin, Pablo
Surprenant, Annmarie
The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title_full The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title_fullStr The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title_full_unstemmed The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title_short The P2X(7) receptor–pannexin connection to dye uptake and IL-1β release
title_sort p2x(7) receptor–pannexin connection to dye uptake and il-1β release
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686830/
https://www.ncbi.nlm.nih.gov/pubmed/19212823
http://dx.doi.org/10.1007/s11302-009-9141-7
work_keys_str_mv AT pelegrinpablo thep2x7receptorpannexinconnectiontodyeuptakeandil1brelease
AT surprenantannmarie thep2x7receptorpannexinconnectiontodyeuptakeandil1brelease
AT pelegrinpablo p2x7receptorpannexinconnectiontodyeuptakeandil1brelease
AT surprenantannmarie p2x7receptorpannexinconnectiontodyeuptakeandil1brelease