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Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery

BACKGROUND: Next-generation sequencing technologies have been applied most often to model organisms or species closely related to a model. However, these methods have the potential to be valuable in many wild organisms, including those of conservation concern. We used Roche 454 pyrosequencing to cha...

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Detalles Bibliográficos
Autores principales: Hale, Matthew C, McCormick, Cory R, Jackson, James R, DeWoody, J Andrew
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688523/
https://www.ncbi.nlm.nih.gov/pubmed/19402907
http://dx.doi.org/10.1186/1471-2164-10-203