High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas

Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both met...

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Detalles Bibliográficos
Autores principales: Parrella, Paola, la Torre, Antonella, Copetti, Massimiliano, Valori, Vanna M., Barbano, Raffaela, Notarangelo, Angelo, Bisceglia, Michele, Gallo, Antonietta Pia, Balsamo, Teresa, Poeta, Maria Luana, Carella, Massimo, Catapano, Domenico, Parisi, Salvatore, Dallapiccola, Bruno, Maiello, Evaristo, D'Angelo, Vincenzo, Fazio, Vito Michele
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688744/
https://www.ncbi.nlm.nih.gov/pubmed/19503806
http://dx.doi.org/10.1155/2009/531692
Descripción
Sumario:Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%–82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.

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