Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei

Genetic manipulation in African trypanosomes typically relies upon electroporation with chromosomal integration of DNA constructs by homologous recombination. Relatively little is known about chromosomal recombination and repair in these organisms however and low transformation efficiency and positi...

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Detalles Bibliográficos
Autores principales: Glover, Lucy, Horn, David
Formato: Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2009
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691778/
https://www.ncbi.nlm.nih.gov/pubmed/19459229
http://dx.doi.org/10.1016/j.molbiopara.2009.03.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691778/
https://www.ncbi.nlm.nih.gov/pubmed/19459229
http://dx.doi.org/10.1016/j.molbiopara.2009.03.010

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