Cargando…
Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei
Genetic manipulation in African trypanosomes typically relies upon electroporation with chromosomal integration of DNA constructs by homologous recombination. Relatively little is known about chromosomal recombination and repair in these organisms however and low transformation efficiency and positi...
| Autores principales: | , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier/North-Holland Biomedical Press
2009
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691778/ https://www.ncbi.nlm.nih.gov/pubmed/19459229 http://dx.doi.org/10.1016/j.molbiopara.2009.03.010 |