Determination of AMP-activated protein kinase phosphorylation sites in recombinant protein expressed using the pET28a vector: A cautionary tale

AMP-activated protein kinase (AMPK) is responsible for sensing of the cell’s energetic status and it phosphorylates numerous substrates involved in anabolic and catabolic processes as well as interacting with signaling cascades. Mutations in the gene encoding the γ2 regulatory subunit have been show...

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Detalles Bibliográficos
Autores principales: Renz, Bernhard, Davies, Joanna K., Carling, David, Watkins, Hugh, Redwood, Charles
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691924/
https://www.ncbi.nlm.nih.gov/pubmed/19269329
http://dx.doi.org/10.1016/j.pep.2009.02.016

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691924/
https://www.ncbi.nlm.nih.gov/pubmed/19269329
http://dx.doi.org/10.1016/j.pep.2009.02.016

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