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Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 p...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Academic Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691925/ https://www.ncbi.nlm.nih.gov/pubmed/19318094 http://dx.doi.org/10.1016/j.exppara.2009.03.010 |
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author | Kiboi, D.M. Irungu, B.N. Langat, B. Wittlin, S. Brun, R. Chollet, J. Abiodun, O. Nganga, J.K. Nyambati, V.C.S. Rukunga, G.M. Bell, A. Nzila, A. |
author_facet | Kiboi, D.M. Irungu, B.N. Langat, B. Wittlin, S. Brun, R. Chollet, J. Abiodun, O. Nganga, J.K. Nyambati, V.C.S. Rukunga, G.M. Bell, A. Nzila, A. |
author_sort | Kiboi, D.M. |
collection | PubMed |
description | We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I(90)) [ED(90) of resistant line/ED(90) of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at −80 °C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem(®)) should not select for PQ resistance. |
format | Text |
id | pubmed-2691925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26919252009-06-11 Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model Kiboi, D.M. Irungu, B.N. Langat, B. Wittlin, S. Brun, R. Chollet, J. Abiodun, O. Nganga, J.K. Nyambati, V.C.S. Rukunga, G.M. Bell, A. Nzila, A. Exp Parasitol Article We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I(90)) [ED(90) of resistant line/ED(90) of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at −80 °C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem(®)) should not select for PQ resistance. Academic Press 2009-07 /pmc/articles/PMC2691925/ /pubmed/19318094 http://dx.doi.org/10.1016/j.exppara.2009.03.010 Text en © 2009 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Kiboi, D.M. Irungu, B.N. Langat, B. Wittlin, S. Brun, R. Chollet, J. Abiodun, O. Nganga, J.K. Nyambati, V.C.S. Rukunga, G.M. Bell, A. Nzila, A. Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title_full | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title_fullStr | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title_full_unstemmed | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title_short | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model |
title_sort | plasmodium berghei anka: selection of resistance to piperaquine and lumefantrine in a mouse model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691925/ https://www.ncbi.nlm.nih.gov/pubmed/19318094 http://dx.doi.org/10.1016/j.exppara.2009.03.010 |
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