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Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model

We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 p...

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Detalles Bibliográficos
Autores principales: Kiboi, D.M., Irungu, B.N., Langat, B., Wittlin, S., Brun, R., Chollet, J., Abiodun, O., Nganga, J.K., Nyambati, V.C.S., Rukunga, G.M., Bell, A., Nzila, A.
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691925/
https://www.ncbi.nlm.nih.gov/pubmed/19318094
http://dx.doi.org/10.1016/j.exppara.2009.03.010
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author Kiboi, D.M.
Irungu, B.N.
Langat, B.
Wittlin, S.
Brun, R.
Chollet, J.
Abiodun, O.
Nganga, J.K.
Nyambati, V.C.S.
Rukunga, G.M.
Bell, A.
Nzila, A.
author_facet Kiboi, D.M.
Irungu, B.N.
Langat, B.
Wittlin, S.
Brun, R.
Chollet, J.
Abiodun, O.
Nganga, J.K.
Nyambati, V.C.S.
Rukunga, G.M.
Bell, A.
Nzila, A.
author_sort Kiboi, D.M.
collection PubMed
description We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I(90)) [ED(90) of resistant line/ED(90) of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at −80 °C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem(®)) should not select for PQ resistance.
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spelling pubmed-26919252009-06-11 Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model Kiboi, D.M. Irungu, B.N. Langat, B. Wittlin, S. Brun, R. Chollet, J. Abiodun, O. Nganga, J.K. Nyambati, V.C.S. Rukunga, G.M. Bell, A. Nzila, A. Exp Parasitol Article We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I(90)) [ED(90) of resistant line/ED(90) of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at −80 °C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem(®)) should not select for PQ resistance. Academic Press 2009-07 /pmc/articles/PMC2691925/ /pubmed/19318094 http://dx.doi.org/10.1016/j.exppara.2009.03.010 Text en © 2009 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Kiboi, D.M.
Irungu, B.N.
Langat, B.
Wittlin, S.
Brun, R.
Chollet, J.
Abiodun, O.
Nganga, J.K.
Nyambati, V.C.S.
Rukunga, G.M.
Bell, A.
Nzila, A.
Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title_full Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title_fullStr Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title_full_unstemmed Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title_short Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model
title_sort plasmodium berghei anka: selection of resistance to piperaquine and lumefantrine in a mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691925/
https://www.ncbi.nlm.nih.gov/pubmed/19318094
http://dx.doi.org/10.1016/j.exppara.2009.03.010
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