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Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection

BACKGROUND: Methodologies like phage display selection, in vitro mutagenesis and the determination of allelic expression differences include steps where large numbers of clones need to be compared and characterised. In the current study we show that high-resolution melt curve analysis (HRMA) is a si...

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Detalles Bibliográficos
Autores principales: Pepers, Barry A, Schut, Menno H, Vossen, Rolf HAM, van Ommen, Gert-Jan B, den Dunnen, Johan T, van Roon-Mom, Willeke MC
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694173/
https://www.ncbi.nlm.nih.gov/pubmed/19463169
http://dx.doi.org/10.1186/1472-6750-9-50