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Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells

BACKGROUND: Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocy...

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Detalles Bibliográficos
Autores principales: Zuccolo, Jonathan, Unruh, Tammy L, Deans, Julie P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695429/
https://www.ncbi.nlm.nih.gov/pubmed/19473530
http://dx.doi.org/10.1186/1471-2172-10-30