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Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells
BACKGROUND: Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocy...
Autores principales: | Zuccolo, Jonathan, Unruh, Tammy L, Deans, Julie P |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695429/ https://www.ncbi.nlm.nih.gov/pubmed/19473530 http://dx.doi.org/10.1186/1471-2172-10-30 |
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