Primary Structure Revision and Active Site Mapping of E. Coli Isoleucyl-tRNA Synthetase by Means of Maldi Mass Spectrometry

The correct amino acid sequence of E. coli isoleucyl-tRNA synthetase (IleRS) was established by means of peptide mapping by MALDI mass spectrometry, using a set of four endoproteases (trypsin, LysC, AspN and GluC). Thereafter, the active site of IleRS was mapped by affinity labeling with reactive an...

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Detalles Bibliográficos
Autores principales: Baouz, Soria, Schmitter, Jean-Marie, Chenoune, Lila, Beauvallet, Christian, Blanquet, Sylvain, Woisard, Anne, Hountondji, Codjo
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695604/
https://www.ncbi.nlm.nih.gov/pubmed/19557155
http://dx.doi.org/10.2174/1874091X00903010026

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695604/
https://www.ncbi.nlm.nih.gov/pubmed/19557155
http://dx.doi.org/10.2174/1874091X00903010026

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