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Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

BACKGROUND: A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS: To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans)...

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Detalles Bibliográficos
Autores principales: Yan, Qitao, Zhao, Rui, Zheng, Wenlin, Yin, Changxin, Zhang, Bao, Ma, Wenli
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2696436/
https://www.ncbi.nlm.nih.gov/pubmed/19457250
http://dx.doi.org/10.1186/1472-6750-9-47