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Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans
BACKGROUND: A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS: To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans)...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2696436/ https://www.ncbi.nlm.nih.gov/pubmed/19457250 http://dx.doi.org/10.1186/1472-6750-9-47 |