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Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus
A bacterial artificial chromosome (BAC) library was constructed by cloning HindIII-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1), while cl...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698005/ https://www.ncbi.nlm.nih.gov/pubmed/14604514 http://dx.doi.org/10.1186/1297-9686-35-7-673 |