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Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins
BACKGROUND: The hot dog fold has been found in more than sixty proteins since the first report of its existence about a decade ago. The fold appears to have a strong association with fatty acid biosynthesis, its regulation and metabolism, as the proteins with this fold are predominantly coenzyme A-b...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698920/ https://www.ncbi.nlm.nih.gov/pubmed/19473548 http://dx.doi.org/10.1186/1472-6807-9-37 |
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author | Pidugu, Lakshmi S Maity, Koustav Ramaswamy, Karthikeyan Surolia, Namita Suguna, Kaza |
author_facet | Pidugu, Lakshmi S Maity, Koustav Ramaswamy, Karthikeyan Surolia, Namita Suguna, Kaza |
author_sort | Pidugu, Lakshmi S |
collection | PubMed |
description | BACKGROUND: The hot dog fold has been found in more than sixty proteins since the first report of its existence about a decade ago. The fold appears to have a strong association with fatty acid biosynthesis, its regulation and metabolism, as the proteins with this fold are predominantly coenzyme A-binding enzymes with a variety of substrates located at their active sites. RESULTS: We have analyzed the structural features and sequences of proteins having the hot dog fold. This study reveals that though the basic architecture of the fold is well conserved in these proteins, significant differences exist in their sequence, nature of substrate and oligomerization. Segments with certain conserved sequence motifs seem to play crucial structural and functional roles in various classes of these proteins. CONCLUSION: The analysis led to predictions regarding the functional classification and identification of possible catalytic residues of a number of hot dog fold-containing hypothetical proteins whose structures were determined in high throughput structural genomics projects. |
format | Text |
id | pubmed-2698920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26989202009-06-19 Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins Pidugu, Lakshmi S Maity, Koustav Ramaswamy, Karthikeyan Surolia, Namita Suguna, Kaza BMC Struct Biol Research Article BACKGROUND: The hot dog fold has been found in more than sixty proteins since the first report of its existence about a decade ago. The fold appears to have a strong association with fatty acid biosynthesis, its regulation and metabolism, as the proteins with this fold are predominantly coenzyme A-binding enzymes with a variety of substrates located at their active sites. RESULTS: We have analyzed the structural features and sequences of proteins having the hot dog fold. This study reveals that though the basic architecture of the fold is well conserved in these proteins, significant differences exist in their sequence, nature of substrate and oligomerization. Segments with certain conserved sequence motifs seem to play crucial structural and functional roles in various classes of these proteins. CONCLUSION: The analysis led to predictions regarding the functional classification and identification of possible catalytic residues of a number of hot dog fold-containing hypothetical proteins whose structures were determined in high throughput structural genomics projects. BioMed Central 2009-05-28 /pmc/articles/PMC2698920/ /pubmed/19473548 http://dx.doi.org/10.1186/1472-6807-9-37 Text en Copyright © 2009 Pidugu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pidugu, Lakshmi S Maity, Koustav Ramaswamy, Karthikeyan Surolia, Namita Suguna, Kaza Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title | Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title_full | Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title_fullStr | Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title_full_unstemmed | Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title_short | Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins |
title_sort | analysis of proteins with the 'hot dog' fold: prediction of function and identification of catalytic residues of hypothetical proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698920/ https://www.ncbi.nlm.nih.gov/pubmed/19473548 http://dx.doi.org/10.1186/1472-6807-9-37 |
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