Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR deg...

Descripción completa

Detalles Bibliográficos
Autores principales: Ponchel, Frederique, Toomes, Carmel, Bransfield, Kieran, Leong, Fong T, Douglas, Susan H, Field, Sarah L, Bell, Sandra M, Combaret, Valerie, Puisieux, Alain, Mighell, Alan J, Robinson, Philip A, Inglehearn, Chris F, Isaacs, John D, Markham, Alex F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC270040/
https://www.ncbi.nlm.nih.gov/pubmed/14552656
http://dx.doi.org/10.1186/1472-6750-3-18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC270040/
https://www.ncbi.nlm.nih.gov/pubmed/14552656
http://dx.doi.org/10.1186/1472-6750-3-18

Ejemplares similares