Construction of siRNA/miRNA expression vectors based on a one-step PCR process

BACKGROUND: RNA interference (RNAi) has become a powerful means for silencing target gene expression in mammalian cells and is envisioned to be useful in therapeutic approaches to human disease. In recent years, high-throughput, genome-wide screening of siRNA/miRNA libraries has emerged as a desirab...

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Detalles Bibliográficos
Autores principales: Xu, Jun, Zeng, Jie Qiong, Wan, Gang, Hu, Gui Bin, Yan, Hong, Ma, Li Xin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700792/
https://www.ncbi.nlm.nih.gov/pubmed/19490634
http://dx.doi.org/10.1186/1472-6750-9-53

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700792/
https://www.ncbi.nlm.nih.gov/pubmed/19490634
http://dx.doi.org/10.1186/1472-6750-9-53

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