Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration

BACKGROUND: Thermostable enzymes from thermophilic microorganisms are playing more and more important roles in molecular biology R&D and industrial applications. However, over-production of recombinant soluble proteins from thermophilic microorganisms in mesophilic hosts (e.g. E. coli) remains c...

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Detalles Bibliográficos
Autores principales: Wang, Yiran, Zhang, Y-H Percival
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701922/
https://www.ncbi.nlm.nih.gov/pubmed/19497097
http://dx.doi.org/10.1186/1475-2859-8-30

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701922/
https://www.ncbi.nlm.nih.gov/pubmed/19497097
http://dx.doi.org/10.1186/1475-2859-8-30

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