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A facile method for expression and purification of the Alzheimer’s disease-associated amyloid β-peptide

We report the development of a high-level bacterial expression system for the Alzheimer’s disease-associated amyloid β-peptide (Aβ), together with a scaleable and inexpensive purification procedure. Aβ(1–40) and Aβ(1–42) coding sequences together with added ATG codons were cloned directly into a Pet...

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Detalles Bibliográficos
Autores principales: Walsh, Dominic M, Thulin, Eva, Minogue, Aedín M, Gustavsson, Niklas, Pang, Eric, Teplow, David B, Linse, Sara
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702495/
https://www.ncbi.nlm.nih.gov/pubmed/19175671
http://dx.doi.org/10.1111/j.1742-4658.2008.06862.x