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A facile method for expression and purification of the Alzheimer’s disease-associated amyloid β-peptide
We report the development of a high-level bacterial expression system for the Alzheimer’s disease-associated amyloid β-peptide (Aβ), together with a scaleable and inexpensive purification procedure. Aβ(1–40) and Aβ(1–42) coding sequences together with added ATG codons were cloned directly into a Pet...
Autores principales: | Walsh, Dominic M, Thulin, Eva, Minogue, Aedín M, Gustavsson, Niklas, Pang, Eric, Teplow, David B, Linse, Sara |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702495/ https://www.ncbi.nlm.nih.gov/pubmed/19175671 http://dx.doi.org/10.1111/j.1742-4658.2008.06862.x |
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