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Determination of quantitative and site-specific DNA methylation of perforin by pyrosequencing

BACKGROUND: Differential expression of perforin (PRF1), a gene with a pivotal role in immune surveillance, can be attributed to differential methylation of CpG sites in its promoter region. A reproducible method for quantitative and CpG site-specific determination of perforin methylation is required...

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Detalles Bibliográficos
Autores principales:	Narasimhan, Supraja, Falkenberg, Virginia R, Khin, Maung M, Rajeevan, Mangalathu S
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704226/ https://www.ncbi.nlm.nih.gov/pubmed/19523225 http://dx.doi.org/10.1186/1756-0500-2-104

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