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A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane

PURPOSE: To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife® culture medium under serum- and feeder-free conditions. METHODS: Cadaveric donor limbal corneal epithelial cells were cultured on denuded amniotic membranes using an explant technique that wa...

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Detalles Bibliográficos
Autores principales: Lekhanont, Kaevalin, Choubtum, Lulin, Chuck, Roy S., Sa-ngiampornpanit, Tarinee, Chuckpaiwong, Varintorn, Vongthongsri, Anun
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704912/
https://www.ncbi.nlm.nih.gov/pubmed/19578552