Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays

BACKGROUND: The development of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that function under identical thermal cycling conditions. The use of a primer optimisation matrix to im...

Descripción completa

Detalles Bibliográficos
Autores principales: Mikeska, Thomas, Dobrovic, Alexander
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706253/
https://www.ncbi.nlm.nih.gov/pubmed/19549292
http://dx.doi.org/10.1186/1756-0500-2-112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706253/
https://www.ncbi.nlm.nih.gov/pubmed/19549292
http://dx.doi.org/10.1186/1756-0500-2-112

Ejemplares similares