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Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays
BACKGROUND: The development of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that function under identical thermal cycling conditions. The use of a primer optimisation matrix to im...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706253/ https://www.ncbi.nlm.nih.gov/pubmed/19549292 http://dx.doi.org/10.1186/1756-0500-2-112 |