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Highly efficient incorporation of the fluorescent nucleotide analogs tC and tC(O) by Klenow fragment
Studies of the mechanisms by which DNA polymerases select the correct nucleotide frequently employ fluorescently labeled DNA to monitor conformational rearrangements of the polymerase–DNA complex in response to incoming nucleotides. For this purpose, fluorescent base analogs play an increasingly imp...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709563/ https://www.ncbi.nlm.nih.gov/pubmed/19401439 http://dx.doi.org/10.1093/nar/gkp266 |