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Highly efficient incorporation of the fluorescent nucleotide analogs tC and tC(O) by Klenow fragment

Studies of the mechanisms by which DNA polymerases select the correct nucleotide frequently employ fluorescently labeled DNA to monitor conformational rearrangements of the polymerase–DNA complex in response to incoming nucleotides. For this purpose, fluorescent base analogs play an increasingly imp...

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Detalles Bibliográficos
Autores principales: Sandin, Peter, Stengel, Gudrun, Ljungdahl, Thomas, Börjesson, Karl, Macao, Bertil, Wilhelmsson, L. Marcus
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709563/
https://www.ncbi.nlm.nih.gov/pubmed/19401439
http://dx.doi.org/10.1093/nar/gkp266