S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-li...

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Detalles Bibliográficos
Autores principales: Peakman, Luke J., Szczelkun, Mark D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Nucleic Acids Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709564/
https://www.ncbi.nlm.nih.gov/pubmed/19401438
http://dx.doi.org/10.1093/nar/gkp267

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709564/
https://www.ncbi.nlm.nih.gov/pubmed/19401438
http://dx.doi.org/10.1093/nar/gkp267

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